Gelatin powder to sheets12/18/2022 However, the above method has faced limitations in dealing with high production costs and ethical and social problems due to the continuous sacrifice of livestock for cell harvesting. To establish a cell line for cultured meat, the initial study adopted a method of separating cells extracted from edible muscle tissue of livestock into muscle satellite cells, adult stem cells, and pluripotent stem cells, followed by culture and proliferation of these cells. It is also important that cell lines are established in an ethical process with minimal or excluded livestock assistance. The ideal cell line for cultured meat production should be available in a continuous supply at reasonable production costs and safety for genetic manipulation or antibiotics. The process of manufacturing cultured meat begins with securing a cell line, including cell extraction and separation. Several global food companies are spurring investments in cultured meat research, and governments are also heeding the call for financial support. Public interest in alternative foods, including cultured meat, is increasing because the global protein supply cannot match the population growth. Cultured meat, known as in-vitro meat or lab-grown meat, is artificial meat produced by the mass culture of stem cells or animal cells without slaughtering livestock. The importance of eco-friendly future foods has been emphasized as voices of the environmental pollution and food crisis due to global warming and an unexpected viral epidemic such as the COVID-19 pandemic. We prepared cultured meats composed of cell sheet containing GMSs and evaluated the quality of the cultured meat by comparing the tissue properties with soy meat and chicken breast. The GMSs exerted four significant functions in the culture of myoblast sheets, and consequently produced cost- and time-effective meat-like cell sheets than the conventional method. We analyzed the loading and release of nutrients for each GMS with diverse surface properties, and selected the most effective GMSs to improve the proliferation of myoblasts under serum-reduced medium. The MAGIC powder had an edible gelatin microsphere (GMS) structure and exhibited different morphologies and bonding activities depending on the degree of crosslinking. Herein, we report on a strategy to develop advanced cultured meat using fish gelatin mass growth-inducing culture (MAGIC) powder and myoblast sheets. The mass proliferation of cells in the cultured meat production is a strenuous process that delays the commercialization of cultured meat because it requires an expensive culture medium for a long period. Cell cultured meat is artificial meat obtained by culturing animal-derived cells in vitro, and received significant attention as an emerging future protein source.
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